Advanced Materials

Shrink-Film Configurable Multiscale Wrinkles for Functional Alignment of Human Embryonic Stem Cells and their Cardiac Derivatives

Authors

  • Aaron Chen,

    1. Department of Chemical Engineering and Material Science, University of California, Irvine, Irvine, CA 92697, USA
    Search for more papers by this author
  • Deborah K. Lieu,

    1. Department of Internal Medicine, Division of Cardiovascular Medicine, University of California, Davis, Davis, CA 95616, USA
    Search for more papers by this author
  • Lauren Freschauf,

    1. Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA
    Search for more papers by this author
  • Valerie Lew,

    1. Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA
    Search for more papers by this author
  • Himanshu Sharma,

    1. Department of Chemical Engineering and Material Science, University of California, Irvine, Irvine, CA 92697, USA
    Search for more papers by this author
  • Jiaxian Wang,

    1. Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong
    2. Cardiovascular Research Center, Mount Sinai School of Medicine, New York, NY 10029, USA
    Search for more papers by this author
  • Diep Nguyen,

    1. Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA
    Search for more papers by this author
  • Ioannis Karakikes,

    1. Cardiovascular Research Center, Mount Sinai School of Medicine, New York, NY 10029, USA
    Search for more papers by this author
  • Roger J. Hajjar,

    1. Cardiovascular Research Center, Mount Sinai School of Medicine, New York, NY 10029, USA
    Search for more papers by this author
  • Ajay Gopinathan,

    1. School of Natural Sciences, University of California, Merced, Merced, CA 95344, USA
    Search for more papers by this author
  • Elliot Botvinick,

    1. Department of Biomedical Engineering, University of California, Irvine, Irvine, CA 92697, USA
    Search for more papers by this author
  • Charless C. Fowlkes,

    1. Department of Computer Science, University of California, Irvine, Irvine, CA 92697, USA
    Search for more papers by this author
  • Ronald A. Li,

    Corresponding author
    1. Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong
    2. Cardiovascular Research Center, Mount Sinai School of Medicine, New York, NY 10029, USA
    3. Hormone & Healthy Aging Research Center, Department of Medicine and Physiology, LKS Faculty of Medicine, University of Hong Kong, Hong Kong
    • Stem Cell & Regenerative Medicine Consortium, LKS Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong
    Search for more papers by this author
  • Michelle Khine

    Corresponding author
    1. Department of Chemical Engineering and Material Science, University of California, Irvine, Irvine, CA 92697, USA
    • Department of Chemical Engineering and Material Science, University of California, Irvine, Irvine, CA 92697, USA.
    Search for more papers by this author

Abstract

A biomimetic substrate for cell-culture is fabricated by plasma treatment of a prestressed thermoplastic shrink film to create tunable multiscaled alignment “wrinkles”. Using this substrate, the functional alignment of human embryonic stem cell derived cardiomyocytes is demonstrated.

original image

Ancillary