Simple Localization of Nanofiber Scaffolds via SU-8 Photoresist and Their Use for Parallel 3D Cellular Assays

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Abstract

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A simple, new approach to localizing electrospun nanofiber scaffolds simply by direct writing of SU-8 photoresist followed by UV polymerization is presented. This method allows 3D cell culture arrays to be produced (see figure) and it can be integrated with microfluidic devices easily to enable low-cost high-throughput cellular assays within an addressable 3D environment, which is attractive for use in drug screening, stem cell research, tissue engineering, and regenerative medicine.

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