Electrospun TiO2 Nanofiber-Based Cell Capture Assay for Detecting Circulating Tumor Cells from Colorectal and Gastric Cancer Patients

Authors

  • Nangang Zhang,

    1. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
    Current affiliation:
    1. These three authors contributed equally to this work.
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  • Yuliang Deng,

    1. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
    Current affiliation:
    1. These three authors contributed equally to this work.
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  • Qidong Tai,

    1. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
    Current affiliation:
    1. These three authors contributed equally to this work.
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  • Boran Cheng,

    1. Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, 430071, PR China
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  • Libo Zhao,

    1. Crump Institute for Molecular Imaging (CIMI), Institute for Molecular Medicine (IMED), Department of Molecular and Medical Pharmacology, California NanoSystems Institute (CNSI), University of California, Los Angeles (UCLA), 570 Westwood Plaza, Building 114, Los Angeles, 90095, California, US
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  • Qinglin Shen,

    1. Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, 430071, PR China
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  • Rongxiang He,

    1. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
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  • Longye Hong,

    1. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
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  • Wei Liu,

    1. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
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  • Shishang Guo,

    Corresponding author
    1. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
    • Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
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  • Kan Liu,

    Corresponding author
    1. College of Electronics and Information Engineering, Wuhan Textile University, Wuhan 430073, Hubei, PR China
    • College of Electronics and Information Engineering, Wuhan Textile University, Wuhan 430073, Hubei, PR China
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  • Hisan-Rong Tseng,

    Corresponding author
    1. Crump Institute for Molecular Imaging (CIMI), Institute for Molecular Medicine (IMED), Department of Molecular and Medical Pharmacology, California NanoSystems Institute (CNSI), University of California, Los Angeles (UCLA), 570 Westwood Plaza, Building 114, Los Angeles, 90095, California, US
    • Crump Institute for Molecular Imaging (CIMI), Institute for Molecular Medicine (IMED), Department of Molecular and Medical Pharmacology, California NanoSystems Institute (CNSI), University of California, Los Angeles (UCLA), 570 Westwood Plaza, Building 114, Los Angeles, 90095, California, US
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  • Bin Xiong,

    Corresponding author
    1. Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, 430071, PR China
    • Zhongnan Hospital of Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan, Hubei, 430071, PR China.
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  • Xing-Zhong Zhao

    Corresponding author
    1. Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
    • Key Laboratory of Artificial Micro- and Nano-Structures of Ministry of Education, School of Physics and Technology, Wuhan University, Wuhan 430072, Hubei, PR China
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Abstract

original image

A nanostructured platform that combines electrospun TiO2 nanofibers (TiNFs)-deposited substrate and cell-capture agent realizes significant capture of circulating tumor cells (CTCs). The enhanced local topographic interactions between the horizontally packed TiNFs deposited substrates and extracellular matrix scaffolds, in addition to anti-EpCAM/EpCAM biological recognition, contributes to the significantly enhanced capture efficiency compared to flat surfaces.

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