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Three-Dimensional Biomimetic Patterning in Hydrogels to Guide Cellular Organization

Authors

  • James C. Culver,

    1. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Joseph C. Hoffmann,

    1. Department of Bioengineering, Rice University, 6100 Main Street, Houston, TX, 77005, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Ross A. Poché,

    1. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA
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  • John H. Slater,

    1. Department of Bioengineering, Rice University, 6100 Main Street, Houston, TX, 77005, USA
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  • Jennifer L. West,

    Corresponding author
    1. Department of Bioengineering, Rice University, 6100 Main Street, Houston, TX, 77005, USA
    • Department of Bioengineering, Rice University, 6100 Main Street, Houston, TX, 77005, USA
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  • Mary E. Dickinson

    Corresponding author
    1. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA
    • Department of Molecular Physiology and Biophysics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA.
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Abstract

An image-guided micropatterning method is demonstrated for generating biomimetic hydrogel scaffolds with two-photon laser scanning photolithography. This process utilizes computational methods to directly translate three-dimensional cytoarchitectural features from labeled tissues into material structures. We use this method to pattern hydrogels that guide cellular organization by structurally and biochemically recapitulating complex vascular niche microenvironments with high pattern fidelity at the microscale.

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