Label-Free Ultrasensitive Detection of Human Telomerase Activity Using Porphyrin-Functionalized Graphene and Electrochemiluminescence Technique

Authors

  • Li Wu,

    1. Division of Biological Inorganic Chemistry, State Key laboratory of Rare Earth Resources Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China, Fax: (+86) 431-85262625
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  • Jiasi Wang,

    1. Division of Biological Inorganic Chemistry, State Key laboratory of Rare Earth Resources Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China, Fax: (+86) 431-85262625
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  • Lingyan Feng,

    1. Division of Biological Inorganic Chemistry, State Key laboratory of Rare Earth Resources Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China, Fax: (+86) 431-85262625
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  • Jinsong Ren,

    1. Division of Biological Inorganic Chemistry, State Key laboratory of Rare Earth Resources Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China, Fax: (+86) 431-85262625
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  • Weili Wei,

    1. Division of Biological Inorganic Chemistry, State Key laboratory of Rare Earth Resources Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China, Fax: (+86) 431-85262625
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  • Xiaogang Qu

    Corresponding author
    1. Division of Biological Inorganic Chemistry, State Key laboratory of Rare Earth Resources Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China, Fax: (+86) 431-85262625
    • Division of Biological Inorganic Chemistry, State Key laboratory of Rare Earth Resources Utilization, Laboratory of Chemical Biology, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China, Fax: (+86) 431-85262625.
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Abstract

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Using porphyrin-functionalized graphene, we construct a PCR-free, low-cost, rapid, and electrochemiluminenscence (ECL) assay for detection of telomerase activity that has been demonstrated in six different cell lines and can be used as initial screening of G-quadruplex DNA binding agents and telomerase inhibitors. This ECL sensor shows highly sensitive for detection of telomerase with the detection limit as low as 10 HeLa cells mL−1.

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