Advanced Materials

Tomography and Static-Mechanical Properties of Adherent Cells

Authors

  • Siowling Soh,

    1. Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA
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  • Kristiana Kandere-Grzybowska,

    Corresponding author
    1. Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA
    • Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA.
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  • Goher Mahmud,

    1. Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA
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  • Sabil Huda,

    1. Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA
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  • Alexander Z. Patashinski,

    1. Department of Chemistry, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA
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  • Bartosz A. Grzybowski

    Corresponding author
    1. Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA
    2. Department of Chemistry, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA
    • Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Rd, Illinois 60208, USA.
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Abstract

A tomography approach is used to reconstruct 3D cell shapes and, simultaneously, the shapes/positions of the nuclei within these cells. Subjecting the cells to well-defined microconfinements of various diameters allow for relating the steady-state shapes of cells to their static-mechanical properties. The observed shapes show striking regularities between different cell types and all fit to a model that takes into account the cell membrane, cortical actin, and the nucleus.

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