Ferritin-based nanoprobe (FBNP) hydrogel provides solutions to some of the traditional problems of bioassays. The schematic shows 3D and highly specific multiplex assays of markers for AIDS and Sjögren's syndrome using quantum-dot based reporters with different fluorescence emissions for the two syndromes. The advantages of using a hydrogel include that the stability of FBNPs is significantly enhanced within the hydrogel, FBNPs are distributed evenly throughout the hydrogel matrix, and the amount of FBNPs within the hydrogel can be well controlled.
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