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Tailoring Substrates for Long-Term Organotypic Culture of Adult Neuronal Tissue

Authors

  • Valentina Dallacasagrande,

    1. Paul-Flechsig-Institute for Brain Research, Universität Leipzig, Jahnallee 59, 04109 Leipzig, Germany
    2. Soft Matter Physics Division, Institut für Experimentelle Physik 1, Universität Leipzig, Linnéstr. 5, 04103 Leipzig, Germany
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Mareike Zink,

    Corresponding author
    1. Soft Matter Physics Division, Institut für Experimentelle Physik 1, Universität Leipzig, Linnéstr. 5, 04103 Leipzig, Germany
    Current affiliation:
    1. These authors contributed equally to this work.
    • Soft Matter Physics Division, Institut für Experimentelle Physik 1, Universität Leipzig, Linnéstr. 5, 04103 Leipzig, Germany
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  • Steven Huth,

    1. Leibniz-Institut für Oberflächenmodifizierung e.V., Permoserstr. 15, 04318 Leipzig, Germany
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  • Alexander Jakob,

    1. Leibniz-Institut für Oberflächenmodifizierung e.V., Permoserstr. 15, 04318 Leipzig, Germany
    2. Translationszentrum für regenerative Medizin/Fakultät für Physik und, Geowissenschaften, Universität Leipzig, Leipzig, Germany
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  • Marcus Müller,

    1. Leibniz-Institut für Oberflächenmodifizierung e.V., Permoserstr. 15, 04318 Leipzig, Germany
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  • Andreas Reichenbach,

    1. Paul-Flechsig-Institute for Brain Research, Universität Leipzig, Jahnallee 59, 04109 Leipzig, Germany
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  • Josef A. Käs,

    1. Soft Matter Physics Division, Institut für Experimentelle Physik 1, Universität Leipzig, Linnéstr. 5, 04103 Leipzig, Germany
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  • S. G. Mayr

    Corresponding author
    1. Leibniz-Institut für Oberflächenmodifizierung e.V., Permoserstr. 15, 04318 Leipzig, Germany
    2. Translationszentrum für regenerative Medizin/Fakultät für Physik und, Geowissenschaften, Universität Leipzig, Leipzig, Germany
    • Leibniz-Institut für Oberflächenmodifizierung e.V., Permoserstr. 15, 04318 Leipzig, Germany.
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Abstract

Organotypic tissue cultures are highly promising for performing in vivo type studies in vitro. Currently, however, very limited survival times of only a few days for adult tissue often severely limit their application. Here, superhydrophilic nanostructured substrates with ideal material properties ensure tissue adhesion, essential for organotypic culture, while migration of single cells out of the tissue is hampered. Tuning substrate properties, for the first time, adult neuronal tissue could be cultured for 14 days with no indications of degeneration.

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