Controlling Spatial Organization of Multiple Cell Types in Defined 3D Geometries

Authors

  • Halil Tekin,

    1. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
    2. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA
    3. David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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  • Jefferson G. Sanchez,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA
    2. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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  • Christian Landeros,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA
    2. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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  • Karen Dubbin,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA
    2. Department of Materials Science and Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
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  • Robert Langer,

    Corresponding author
    1. David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
    2. Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
    3. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
    • David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
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  • Ali Khademhosseini

    Corresponding author
    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA
    2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA
    • Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA
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Abstract

original image

A simple method to control spatial organizations of multiple cell types in predefined geometries by using poly(N-isopropylacrylamide)-based dynamic microwells is described. Dynamic microwells are used to pattern two different cell types in a spatially controlled manner by exploiting their shape changing properties at two different temperatures. Both spatial organization of two different cell types and control of pattern geometry are achieved with circular and square dynamic microwells.

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