Real-Time, Sensitive, and Specific Detection of Promoter-Polymerase Interactions in Gene Transcription Using a Nanoplasmonic Sensor

Authors

  • Min Sun Song,

    1. Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea
    Current affiliation:
    1. These authors contributed equally to this work.
    Search for more papers by this author
  • Seung Phill Choi,

    1. Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 136-701, Korea
    Current affiliation:
    1. These authors contributed equally to this work.
    Search for more papers by this author
  • Jeewon Lee,

    1. Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 136-701, Korea
    Search for more papers by this author
  • Young Jik Kwon,

    1. Department of Pharmaceutical Sciences and Chemical Engineering & Materials Science, University of California, Irvine, Irvine, CA 92697, USA
    Search for more papers by this author
  • Sang Jun Sim

    Corresponding author
    1. Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 136-701, Korea
    • Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 136-701, Korea.
    Search for more papers by this author

Abstract

original image

Real-time, sensitive and specific analysis of gene transcription: The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. We can simply detect which base pair of point-mutated promoter is important for the specific recognition by RNA polymerase. This is the first attempt to analyze transcription activity, based on the optical property of single gold nanoparticle.

Ancillary