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Keywords:

  • DNA-protein interaction;
  • nanoplasmonic biosensor;
  • real-time analysis;
  • promoter activity;
  • gene transcription
Thumbnail image of graphical abstract

Real-time, sensitive and specific analysis of gene transcription: The interactions between promoter and RNA polymerase that lead to gene transcription are monitored using Rayleigh light scattering of gold nanoparticle. We can simply detect which base pair of point-mutated promoter is important for the specific recognition by RNA polymerase. This is the first attempt to analyze transcription activity, based on the optical property of single gold nanoparticle.