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Decoupling Cell and Matrix Mechanics in Engineered Microtissues Using Magnetically Actuated Microcantilevers

Authors

  • Ruogang Zhao,

    1. Department of Physics and Astronomy, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, USA 21218
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  • Thomas Boudou,

    1. Department of Bioengineering, University of Pennsylvania, 510 Skirkanich Hall, 210 South 33rd Street, Philadelphia, PA, USA 19104
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  • Wei-Gang Wang,

    1. Department of Physics and Astronomy, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, USA 21218
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  • Christopher S. Chen,

    Corresponding author
    1. Department of Bioengineering, University of Pennsylvania, 510 Skirkanich Hall, 210 South 33rd Street, Philadelphia, PA, USA 19104
    • Department of Bioengineering, University of Pennsylvania, 510 Skirkanich Hall, 210 South 33rd Street, Philadelphia, PA, USA 19104.
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  • Daniel H. Reich

    Corresponding author
    1. Department of Physics and Astronomy, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, USA 21218
    • Department of Physics and Astronomy, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, USA 21218
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Abstract

A novel bio-magnetomechanical microtissue system is described for magnetic actuation of arrays of 3D microtissues using microcantilevers. This system enables both in situ measurements of fundamental mechanical properties of engineered tissue, such as contractility and stiffness, as well as dynamic stimulation of the microtissues. Using this system, cell and extracellular matrix contributions to the tissue mechanical properties are decoupled for the first time under both static and dynamic loading conditions.

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