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Communication

Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes

  1. Christopher Schirwitz1,*,
  2. Felix F. Loeffler1,
  3. Thomas Felgenhauer2,
  4. Volker Stadler2,
  5. Alexander Nesterov-Mueller3,
  6. Reiner Dahint4,
  7. Frank Breitling3,
  8. F. Ralf Bischoff1

Article first published online: 14 JAN 2013

DOI: 10.1002/adma.201203853

Issue

Advanced Materials

Advanced Materials

Volume 25, Issue 11, pages 1598–1602, March 20, 2013

Additional Information

How to Cite

Schirwitz, C., Loeffler, F. F., Felgenhauer, T., Stadler, V., Nesterov-Mueller, A., Dahint, R., Breitling, F. and Bischoff, F. R. (2013), Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes. Adv. Mater., 25: 1598–1602. doi: 10.1002/adma.201203853

Author Information

  1. 1

    German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany

  2. 2

    PEPperPRINT GmbH, Rischerstrasse 12, 69123 Heidelberg, Germany

  3. 3

    Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Peptide Arrays; Hermann-von-Helmholtz-Platz, 1, 76344 Eggenstein-Leopoldshafen, Germany

  4. 4

    University of Heidelberg, Applied Physical Chemistry, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany

*German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.

Publication History

  1. Issue published online: 11 MAR 2013
  2. Article first published online: 14 JAN 2013
  3. Manuscript Revised: 22 NOV 2012
  4. Manuscript Received: 14 SEP 2012

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Keywords:

  • peptide microarrays;
  • purification;
  • high-complexity;
  • high-density;
  • microchips;
  • laser printers
Thumbnail image of graphical abstract

A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm2 is demonstrated.

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