Advanced Materials

Purification of High-Complexity Peptide Microarrays by Spatially Resolved Array Transfer to Gold-Coated Membranes

Authors

  • Christopher Schirwitz,

    Corresponding author
    1. German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    • German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany.
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  • Felix F. Loeffler,

    1. German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
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  • Thomas Felgenhauer,

    1. PEPperPRINT GmbH, Rischerstrasse 12, 69123 Heidelberg, Germany
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  • Volker Stadler,

    1. PEPperPRINT GmbH, Rischerstrasse 12, 69123 Heidelberg, Germany
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  • Alexander Nesterov-Mueller,

    1. Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Peptide Arrays; Hermann-von-Helmholtz-Platz, 1, 76344 Eggenstein-Leopoldshafen, Germany
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  • Reiner Dahint,

    1. University of Heidelberg, Applied Physical Chemistry, Im Neuenheimer Feld 253, 69120 Heidelberg, Germany
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  • Frank Breitling,

    1. Karlsruhe Institute of Technology (KIT), Institute of Microstructure Technology (IMT), Peptide Arrays; Hermann-von-Helmholtz-Platz, 1, 76344 Eggenstein-Leopoldshafen, Germany
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  • F. Ralf Bischoff

    1. German Cancer Research Center (DKFZ), Functional Genome Analysis, Chip-based Peptide Libraries, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
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Abstract

A method for the one-step purification of high-complexity peptide microarrays is presented. The entire peptide library is transferred from the synthesis support to a gold coated polyvinylidenfluoride (PVDF) membrane, whereby only full-length peptides covalently couple to the receptor membrane via an N-terminally added cysteine. Highly resolved peptide transfer and purification of up to 10 000 features per cm2 is demonstrated.

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