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Engineered Large Spider Eggcase Silk Protein for Strong Artificial Fibers

Authors

  • Zhi Lin,

    1. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543
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  • Qinqiu Deng,

    1. Department of Physics, National University of Singapore, 2 Science Drive 3, Singapore 117551, NUS Graduate School for Integrative Sciences and Engineering, Singapore, 117456
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  • Xiang-Yang Liu,

    1. Department of Physics, National University of Singapore, 2 Science Drive 3, Singapore 117551, NUS Graduate School for Integrative Sciences and Engineering, Singapore, 117456
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  • Daiwen Yang

    Corresponding author
    1. Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543
    • Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543.
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Abstract

A simple strategy is developed to produce large silk proteins in commercially available E. coli. The strategy is demonstrated for the production of a modified eggcase silk protein with a molecular weight of ∼378 kDa. Using a non-toxic water coagulation bath developed here, the recombinant protein is spun into artificial fibers that reach higher tenacity than its native counterpart. The strategy is generally applicable to synthesizing other types of large silk proteins with tandem repetitive domains.

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