Triggering Cell Adhesion, Migration or Shape Change with a Dynamic Surface Coating

Authors

  • Stijn F. M. van Dongen,

    Corresponding author
    1. École Normale Supérieure, Department of chemistry, UMR 8640 CNRS-ENS-UPMC, 24 rue Lhomond, 75005, Paris, France
    • École Normale Supérieure, Department of chemistry, UMR 8640 CNRS-ENS-UPMC, 24 rue Lhomond, 75005, Paris, France
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  • Paolo Maiuri,

    1. Institut Curie, Team Systems Biology of Cell Division and Cell Polarity, UMR 144 IC-CNRS, 26 rue d'Ulm, 75248 Cedex 05, Paris, France
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  • Emmanuelle Marie,

    1. École Normale Supérieure, Department of chemistry, UMR 8640 CNRS-ENS-UPMC, 24 rue Lhomond, 75005, Paris, France
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  • Christophe Tribet,

    1. École Normale Supérieure, Department of chemistry, UMR 8640 CNRS-ENS-UPMC, 24 rue Lhomond, 75005, Paris, France
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  • Matthieu Piel

    Corresponding author
    1. Institut Curie, Team Systems Biology of Cell Division and Cell Polarity, UMR 144 IC-CNRS, 26 rue d'Ulm, 75248 Cedex 05, Paris, France
    • Institut Curie, Team Systems Biology of Cell Division and Cell Polarity, UMR 144 IC-CNRS, 26 rue d'Ulm, 75248 Cedex 05, Paris, France.
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Abstract

original image

There's an APP for that: cell-repellent APP (azido-[polylysine-g-PEG]) is used to create substrates for spatially controlled dynamic cell adhesion. The simple addition of a functional peptide to the culture medium rapidly triggers cell adhesion. This highly accessible yet powerful technique allows diverse applications, demonstrated through tissue motility assays, patterned coculturing and triggered cell shape change.

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