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Advanced Materials

High-Purity Prostate Circulating Tumor Cell Isolation by a Polymer Nanofiber-Embedded Microchip for Whole Exome Sequencing

Authors

  • Libo Zhao,

    1. Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Science, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China
    2. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Yi-Tsung Lu,

    1. Urologic Oncology Program & Uro-Oncology Research Program, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Los Angeles, California 90048, USA
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Fuqiang Li,

    1. BGI-ShenZhen, 2nd F, Building No. 11, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Kui Wu,

    1. BGI-ShenZhen, 2nd F, Building No. 11, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China
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  • Shuang Hou,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
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  • Juehua Yu,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
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  • Qinglin Shen,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
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  • Dongxia Wu,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
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  • Min Song,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
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  • Wei-Han OuYang,

    1. CytoLumina Technologies Corp., 21038 Commerce Point Dr., Walnut, California 91789, USA
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  • Zheng Luo,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
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  • Tom Lee,

    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
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  • Xiaohong Fang,

    1. Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Science, Beiyi Street 2#, Zhongguancun, Beijing, 100190, P. R. China
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  • Chen Shao,

    1. Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, China
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  • Xun Xu,

    Corresponding author
    1. BGI-ShenZhen, 2nd F, Building No. 11, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China
    • BGI-ShenZhen, 2nd F, Building No. 11, Beishan Industrial Zone, Yantian District, Shenzhen 518083, China
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  • Mitch A. Garcia,

    Corresponding author
    1. CytoLumina Technologies Corp., 21038 Commerce Point Dr., Walnut, California 91789, USA
    • CytoLumina Technologies Corp., 21038 Commerce Point Dr., Walnut, California 91789, USA.
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  • Leland W. K. Chung,

    Corresponding author
    1. Urologic Oncology Program & Uro-Oncology Research Program, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Los Angeles, California 90048, USA
    • Urologic Oncology Program & Uro-Oncology Research Program, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Los Angeles, California 90048, USA
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  • Matthew Rettig,

    Corresponding author
    1. Departments of Medicine and Urology, Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Chief, Division of Hematology-Oncology, VA Greater Los Angeles Healthcare System
    • Departments of Medicine and Urology, Jonsson Comprehensive Cancer Center, University of California at Los Angeles, Chief, Division of Hematology-Oncology, VA Greater Los Angeles Healthcare System
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  • Hsian-Rong Tseng,

    Corresponding author
    1. Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
    • Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging (CIMI), California NanoSystems Institute (CNSI), University of California at Los Angeles, 570 Westwood Plaza, Build 114, Los Angeles, California 90095-1770, USA
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  • Edwin M. Posadas

    Corresponding author
    1. Urologic Oncology Program & Uro-Oncology Research Program, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Los Angeles, California 90048, USA
    • Urologic Oncology Program & Uro-Oncology Research Program, Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, 8700 Beverly Blvd., Los Angeles, California 90048, USA
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Abstract

Handpick single cancer cells: A modified NanoVelcro Chip is coupled with ArcturusXT laser capture microdissection (LCM) technology to enable the detection and isolation of single circulating tumor cells (CTCs) from patients with prostate cancer (PC). This new approach paves the way for conducting next-generation sequencing (NGS) on single CTCs.

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