A New Diamond Biosensor with Integrated Graphitic Microchannels for Detecting Quantal Exocytic Events from Chromaffin Cells

Authors

  • Federico Picollo,

    Corresponding author
    1. Department of Physics, NIS Centre of Excellence, CNISM Research Unit - University of Torino, INFN Sez. Torino, via P. Giuria 1, Torino, 10125, Italy
    Current affiliation:
    1. These authors contributed equally to this work.
    • Department of Physics, NIS Centre of Excellence, CNISM Research Unit - University of Torino, INFN Sez. Torino, via P. Giuria 1, Torino, 10125, Italy.
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  • Sara Gosso,

    1. Department of Drug Science and Technology, NIS Centre of Excellence, CNISM Research Unit - University of Torino, Corso Raffaello 30, Torino, 10125, Italy
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Ettore Vittone,

    1. Department of Physics, NIS Centre of Excellence, CNISM Research Unit - University of Torino, INFN Sez. Torino, via P. Giuria 1, Torino, 10125, Italy
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  • Alberto Pasquarelli,

    1. Institute of Electron Devices and Circuits, Ulm University, Albert Einstein Allee 45, Ulm, 89069, Germany
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  • Emilio Carbone,

    1. Department of Drug Science and Technology, NIS Centre of Excellence, CNISM Research Unit - University of Torino, Corso Raffaello 30, Torino, 10125, Italy
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  • Paolo Olivero,

    1. Department of Physics, NIS Centre of Excellence, CNISM Research Unit - University of Torino, INFN Sez. Torino, via P. Giuria 1, Torino, 10125, Italy
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  • Valentina Carabelli

    1. Department of Drug Science and Technology, NIS Centre of Excellence, CNISM Research Unit - University of Torino, Corso Raffaello 30, Torino, 10125, Italy
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Abstract

original image

An MeV ion-microbeam lithographic technique can be successfully employed for the fabrication of an all-carbon miniaturized cellular biosensor based on graphitic microchannels embedded in a single-crystal diamond matrix. The device is functionally characterized for the in vitro recording of quantal exocytic events from single chromaffin cells, with high sensitivity and signal-to-noise ratio, opening promising perspectives for the realization of monolithic all-carbon cellular biosensors.

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