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Advanced Materials

3D Structural Patterns in Scalable, Elastomeric Scaffolds Guide Engineered Tissue Architecture

Authors

  • Martin E. Kolewe,

    1. Harvard-MIT Division of Health Sciences and Technology, David H. Koch Institute for Integrative Cancer Research, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
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  • Hyoungshin Park,

    1. Microsystems Development and Microfabrication, Process Engineering Groups, Charles Stark Draper Laboratory, Cambridge, MA 02139, USA
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  • Caprice Gray,

    1. Microsystems Development and Microfabrication, Process Engineering Groups, Charles Stark Draper Laboratory, Cambridge, MA 02139, USA
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  • Xiaofeng Ye,

    1. Harvard-MIT Division of Health Sciences and Technology, David H. Koch Institute for Integrative Cancer Research, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
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  • Robert Langer,

    1. Harvard-MIT Division of Health Sciences and Technology, David H. Koch Institute for Integrative Cancer Research, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
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  • Lisa E. Freed

    Corresponding author
    1. Harvard-MIT Division of Health Sciences and Technology, David H. Koch Institute for Integrative Cancer Research, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA
    2. Microsystems Development and Microfabrication, Process Engineering Groups, Charles Stark Draper Laboratory, Cambridge, MA 02139, USA
    • Harvard-MIT Division of Health Sciences and Technology, David H. Koch Institute for Integrative Cancer Research, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
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  • Dedicated to the memory of Officer Sean Collier, for his caring service to the MIT community and for his sacrifice

Abstract

Microfabricated elastomeric scaffolds with 3D structural patterns are created by semiautomated layer-by-layer assembly of planar polymer sheets with through-pores. The mesoscale interconnected pore architectures governed by the relative alignment of layers are shown to direct cell and muscle-like fiber orientation in both skeletal and cardiac muscle, enabling scale up of tissue constructs towards clinically relevant dimensions.

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