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Advanced Materials

Cell-laden Microengineered and Mechanically Tunable Hybrid Hydrogels of Gelatin and Graphene Oxide

Authors

  • Su Ryon Shin,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA., Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
    2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA
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    • [+]Present Address: Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Korea

    • [+]These authors contributed equally to this work.

  • Behnaz Aghaei-Ghareh-Bolagh,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA., Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
    2. Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden
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    • [+]Present Address: Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Korea

    • [+]These authors contributed equally to this work.

  • Tram T. Dang,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA
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  • Seda Nur Topkaya,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA., Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
    2. Department of Analytical Chemistry, Faculty of Pharmacy, Ege University, Izmir, Turkey
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  • Xiguang Gao,

    1. Department of Chemistry & Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, Canada
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  • Seung Yun Yang,

    1. Department of Medicine, Brigham and Women's Hospital, Cambridge, MA, USA
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    • [++]Present Address: Department of Biomaterial Science, College of Natural Resources 8 Life Science, Pusan National University, Pusan 627-706, Korea

  • Sung Mi Jung,

    1. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA
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  • Jong Hyun Oh,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA., Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
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    • [+]Present Address: Division of Mechanical Design Engineering, Chonbuk National University, Jeonju 561-756, Korea

    • [+]These authors contributed equally to this work.

  • Mehmet R. Dokmeci,

    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA., Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
    2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA
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  • Xiaowu (Shirley) Tang,

    Corresponding author
    1. Department of Chemistry & Waterloo Institute for Nanotechnology, University of Waterloo, Waterloo, Ontario, Canada
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  • Ali Khademhosseini

    Corresponding author
    1. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA., Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, USA
    2. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA
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Abstract

Incorporating graphene oxide inside GelMA hydrogels enhances their mechanical properties and reduces UV-induced cell damage while preserving their favorable characteristics for 3D cell encapsulation. NIH-3T3 fibroblasts encapsulated in GO-GelMA microgels demonstrate excellent cellular viability, proliferation, spreading, and alignment. GO reinforcement combined with a multi-stacking approach offers a facile engineering strategy for the construction of complex artificial tissues.

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