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Advanced Materials

A Triggered DNA Hydrogel Cover to Envelop and Release Single Cells

Authors

  • Juan Jin,

    1. Key Laboratory of Organic Optoelectrics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China, Fax: +86-10-62796082
    2. National Center for Nanoscience and Technology, Beijing 100190, P. R. China
    3. University of Chinese Academy of Sciences, Beijing 100049, P. R. China
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  • Yongzheng Xing,

    1. Key Laboratory of Organic Optoelectrics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China, Fax: +86-10-62796082
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  • Yanli Xi,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China, Fax: +86-10-82627566
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  • Xueli Liu,

    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China, Fax: +86-10-82627566
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  • Tao Zhou,

    1. Key Laboratory of Organic Optoelectrics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China, Fax: +86-10-62796082
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  • Xinxin Ma,

    1. Key Laboratory of Organic Optoelectrics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China, Fax: +86-10-62796082
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  • Zhongqiang Yang,

    1. Key Laboratory of Organic Optoelectrics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China, Fax: +86-10-62796082
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  • Shutao Wang,

    Corresponding author
    1. Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China, Fax: +86-10-82627566
    • Beijing National Laboratory for Molecular Sciences (BNLMS), Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R. China, Fax: +86-10-82627566.
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  • Dongsheng Liu

    Corresponding author
    1. Key Laboratory of Organic Optoelectrics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China, Fax: +86-10-62796082
    • Key Laboratory of Organic Optoelectrics & Molecular Engineering of the Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China, Fax: +86-10-62796082
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Abstract

We develop an enzyme-triggered permeable DNA hydrogel cover to envelop and release single cells in microwells. The porous structure of the DNA hydrogel allows nutrients and waste to pass through, leading to a cell viability as high as 98%. The design provides a general method to culture, monitor, and manipulate single cells, and has potential applications in cell patterning and studying cell communication.

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