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Advanced Materials

Biophysically Defined and Cytocompatible Covalently Adaptable Networks as Viscoelastic 3D Cell Culture Systems

Authors

  • Daniel D. McKinnon,

    1. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
    2. BioFrontiers Institute, University of Colorado Boulder, Boulder, CO
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  • Dylan W. Domaille,

    1. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
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  • Jennifer N. Cha,

    1. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
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  • Kristi S. Anseth

    Corresponding author
    1. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
    2. BioFrontiers Institute, University of Colorado Boulder, Boulder, CO
    3. Howard Hughes Medical Institute University of Colorado Boulder, Boulder, CO
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Abstract

Presented here is a cytocompatible covalently adaptable hydrogel uniquely capable of mimicking the complex biophysical properties of native tissue and enabling natural cell functions without matrix degradation. Demonstrated is both the ability to control elastic modulus and stress relaxation time constants by more than an order of magnitude while predicting these values based on fundamental theoretical understanding and the simulation of muscle tissue and the encapsulation of myoblasts.

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