Micropatterned Polymeric Nanosheets for Local Delivery of an Engineered Epithelial Monolayer

Authors

  • Toshinori Fujie,

    1. WPI-Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Aoba-ku, Sendai, Japan
    2. Department of Life Science and Medical Bioscience, School of Advanced Science and Engineering, Waseda University, Shinjuku-ku, Tokyo, Japan
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  • Yoshihiro Mori,

    1. Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai, Japan
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  • Shuntaro Ito,

    1. Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai, Japan
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  • Matsuhiko Nishizawa,

    1. Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai, Japan
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  • Hojae Bae,

    1. College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, Kwangjin-gu, Seoul, Republic of Korea
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  • Nobuhiro Nagai,

    1. Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan
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  • Hideyuki Onami,

    1. Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan
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  • Toshiaki Abe,

    1. Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, Aoba-ku, Sendai, Japan
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  • Ali Khademhosseini,

    Corresponding author
    1. WPI-Advanced Institute for Materials Research (WPI-AIMR), Tohoku University, Aoba-ku, Sendai, Japan
    2. Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Wyss Institute for Biologically Inspired Engineering, Harvard University, Cambridge, MA, USA
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  • Hirokazu Kaji

    Corresponding author
    1. Department of Bioengineering and Robotics, Graduate School of Engineering, Tohoku University, Aoba-ku, Sendai, Japan
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Abstract

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Like a carpet for cells, micropatterned polymeric nanosheets are developed toward local cell delivery. The nanosheets direct morphogenesis of retinal pigment epithelial (RPE) cells and allow for the injection of an engineered RPE monolayer through syringe needles without the loss of cell viability. Such an ultrathin carrier has the promise of a minimally invasive delivery of cells into narrow tissue spaces.

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