Sensors: Real-Time, Sensitive, and Specific Detection of Promoter-Polymerase Interactions in Gene Transcription Using a Nanoplasmonic Sensor (Adv. Mater. 9/2013)

Authors

  • Min Sun Song,

    1. Department of Chemical Engineering, Sungkyunkwan University, Suwon 440-746, Korea
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Seung Phill Choi,

    1. Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 136-701, Korea
    Current affiliation:
    1. These authors contributed equally to this work.
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  • Jeewon Lee,

    1. Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 136-701, Korea
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  • Young Jik Kwon,

    1. Department of Pharmaceutical Sciences and Chemical Engineering & Materials Science, University of California, Irvine, Irvine, CA 92697, USA
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  • Sang Jun Sim

    Corresponding author
    1. Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 136-701, Korea
    • Department of Chemical and Biological Engineering and Green School, Korea University, Seoul 136-701, Korea.
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Abstract

original image

Sang Jun Sim and co-workers demonstrate on page 1265 the specific recognition of pointmutated promoter by RNA polymerase. The interactions between promoter and RNA polymerase are monitored using Rayleigh light scattering on gold nanoparticles. Using a darkfield microscope, the individual gold nanoparticles can be visualized as the bright colored spots (red, green, or orange in the cover image) by light scattering. Binding of RNA polymerase on the surface of a AuNP is quantitatively detected by LSPR (localized surface plasmon resonance) maximum wavelength shift.

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