Advanced Materials

Hydrogels: Biophysically Defined and Cytocompatible Covalently Adaptable Networks as Viscoelastic 3D Cell Culture Systems (Adv. Mater. 6/2014)

Authors

  • Daniel D. McKinnon,

    1. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
    2. BioFrontiers Institute, University of Colorado Boulder, Boulder, CO
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  • Dylan W. Domaille,

    1. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
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  • Jennifer N. Cha,

    1. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
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  • Kristi S. Anseth

    Corresponding author
    1. Department of Chemical and Biological Engineering, University of Colorado Boulder, Boulder, CO
    2. BioFrontiers Institute, University of Colorado Boulder, Boulder, CO
    3. Howard Hughes Medical Institute University of Colorado Boulder, Boulder, CO
    • Department of Chemical and Biological Engineering, University of Colorado Boulder, Jennie Smoly Caruthers Biotechnology Building, 3415 Colorado Ave, 596 UCB, Boulder CO 80303

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Abstract

A cell stressing a hydrazone-crosslinked, step-growth covalently adaptable poly(ethylene) glycol polymer network is shown in the image. Because of the dynamic nature of the hydrazone bonds, the stressed crosslinks yield to the forces applied by the cytoskeleton, allowing the cell to spread without perturbing the biophysical properties of the cellular microenvironment, as shown by Kristi S. Anseth and co-workers on page 865.

image

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