Small Photoblinking Semiconductor Polymer Dots for Fluorescence Nanoscopy

Authors

  • Xuanze Chen,

    1. State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China
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  • Rongqin Li,

    1. State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China
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  • Zhihe Liu,

    1. State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, China
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  • Kai Sun,

    1. State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, China
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  • Zezhou Sun,

    1. State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, China
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  • Danni Chen,

    1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China
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  • Gaixia Xu,

    1. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China
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  • Peng Xi,

    1. State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China
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  • Changfeng Wu,

    Corresponding author
    1. State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University, Changchun, China
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  • Yujie Sun

    Corresponding author
    1. State Key Laboratory of Membrane Biology, Biodynamic Optical Imaging Center (BIOPIC), School of Life Sciences, and Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China
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Abstract

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Two types of small photoblinking Pdots with high brightness, strong photostability, and favorable biocompatibility, are designed. Super-resolution optical fluctuation imaging is achieved using these Pdots. Imaging of subcellular structures demonstrates that these small photoblinking Pdots are outstanding probes for fast, long-term super-resolution fluorescence imaging.

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