A Thermostable Aldolase for the Synthesis of 3-Deoxy-2-ulosonic Acids



A stereochemically promiscuous 2-keto-3-deoxygluconate aldolase has been used as an efficient biocatalyst to catalyse the aldol reaction of pyruvate with C3- and C4-aldoses to afford syn- and anti-3-deoxy-2-ulosonic acids in poor to good de. A continuous flow bioreactor containing immobilised aldolase has been developed that enables gram quantities of C6- and C7-3-deoxyhept-2-ulosonic acids to be produced in an efficient manner.