Efficient Whole-Cell Biocatalytic Synthesis of N-Acetyl-D-neuraminic Acid

Authors

  • Ping Xu,

    1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China, Fax: (+86)-531-8856-7250
    2. Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China
    Search for more papers by this author
  • Jian Hua Qiu,

    1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China, Fax: (+86)-531-8856-7250
    Search for more papers by this author
    • J. H. Qiu, Y. N. Zhang and J. Chen contributed equally to this work.

  • Yi Nan Zhang,

    1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China, Fax: (+86)-531-8856-7250
    Search for more papers by this author
    • J. H. Qiu, Y. N. Zhang and J. Chen contributed equally to this work.

  • Jing Chen,

    1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China, Fax: (+86)-531-8856-7250
    Search for more papers by this author
    • J. H. Qiu, Y. N. Zhang and J. Chen contributed equally to this work.

  • Peng George Wang,

    1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China, Fax: (+86)-531-8856-7250
    Search for more papers by this author
  • Bing Yan,

    1. School of Pharmaceutical Sciences, Shandong University, Jinan 250100, Shandong Province, People's Republic of China
    Search for more papers by this author
  • Jing Song,

    1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China, Fax: (+86)-531-8856-7250
    Search for more papers by this author
  • Ri Mo Xi,

    1. Department of Chemistry, Shandong University, Jinan 250100, Shandong Province, People's Republic of China
    Search for more papers by this author
  • Zi Xin Deng,

    1. Key Laboratory of Microbial Metabolism, Ministry of Education, College of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic of China
    Search for more papers by this author
  • Cui Qing Ma

    1. State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, Shandong Province, People's Republic of China, Fax: (+86)-531-8856-7250
    Search for more papers by this author

Abstract

N-Acetyl-D-neuraminic acid (Neu5Ac) was efficiently synthesized from lactate and a mixture of N-acetyl-D-glucosamine (GlcNAc) and N-acetyl-D-mannosamine (ManNAc) by whole cells. The biotransformation utilized Escherichia coli cells (Neu5Ac aldolase), Pseudomonas stutzeri cells (lactate oxidase components), GlcNAc/ManNAc and lactate. By this process, 18.32±0.56 g/liter Neu5Ac were obtained from 65.61±2.70 g/liter lactate as an initial substrate input. Neu5Ac (98.4±0.4 % purity, 80.87±0.79 % recovery yield) was purified by anionic exchange chromatography. Our results demonstrate that the reported Neu5Ac biosynthetic process can compare favorably with natural product extraction or chemical synthesis processes.

Ancillary