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Molecularly Ordered Bioelectrocatalytic Composite Inside a Film of Aligned Carbon Nanotubes

Authors

  • Syuhei Yoshino,

    1. Department of Bioengineering and Robotics, Tohoku University, 6-6-1 Aoba, Sendai 980-8579, Japan
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  • Takeo Miyake,

    1. Department of Bioengineering and Robotics, Tohoku University, 6-6-1 Aoba, Sendai 980-8579, Japan
    2. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo 102-0075, Japan
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  • Takeo Yamada,

    1. National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 308-8565, Japan
    2. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo 102-0075, Japan
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  • Kenji Hata,

    1. National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 308-8565, Japan
    2. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo 102-0075, Japan
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  • Matsuhiko Nishizawa

    Corresponding author
    1. Department of Bioengineering and Robotics, Tohoku University, 6-6-1 Aoba, Sendai 980-8579, Japan
    2. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), Tokyo 102-0075, Japan
    • Department of Bioengineering and Robotics, Tohoku University, 6-6-1 Aoba, Sendai 980-8579, Japan.
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Abstract

Molecularly ordered composites of polyvinylimidazole-[Os(bipyridine)2Cl] (PVI-[Os(bpy)2Cl]) and glucose oxidase (GOD) are assembled inside a film of aligned carbon nanotubes. The structure of the prepared GOD/PVI-[Os(bpy)2Cl]/CNT composite film is entirely uniform and stable; more than 90% bioelectrocatalytic activity could be maintained even after storage for 6 d. Owing to the ideal positional relationship achieved between enzyme, mediator, and electrode, the prepared film shows a high bioelectrocatalytic activity for glucose oxidation (ca. 15 mA cm−2 at 25 °C) with an extremely high electron-transfer turnover rate (ca. 650 s−1) comparable to the value for GOD solutions, indicating almost every enzyme molecule entrapped within the ensemble (ca. 3 × 1012 enzymes in a 1 mm × 1 mm film) can work to the fullest extent. This free-standing, flexible composite film can be used by winding on a needle device; as an example, a self-powered sugar monitor is demonstrated.

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