Separations

Novel membrane processes for the enantiomeric resolution of tryptophan by selective permeation enhancements

  1. Zhengzhong Zhou1,
  2. Youchang Xiao2,
  3. T. Alan Hatton3,
  4. Tai-Shung Chung4,*

Article first published online: 21 JUN 2010

DOI: 10.1002/aic.12336

Issue

AIChE Journal

AIChE Journal

Volume 57, Issue 5, pages 1154–1162, May 2011

Additional Information

How to Cite

Zhou, Z., Xiao, Y., Hatton, T. A. and Chung, T.-S. (2011), Novel membrane processes for the enantiomeric resolution of tryptophan by selective permeation enhancements. AIChE J., 57: 1154–1162. doi: 10.1002/aic.12336

Author Information

  1. 1

    Chemical & Pharmaceutical Engineering, Singapore-MIT Alliance, National University of Singapore, Singapore 119260, Singapore

  2. 2

    Dept. of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 119260, Singapore

  3. 3

    Dept. of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139

  4. 4

    Dept. of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 119260, Singapore

*Dept. of Chemical & Biomolecular Engineering, National University of Singapore, Singapore 119260, Singapore

Publication History

  1. Issue published online: 12 APR 2011
  2. Article first published online: 21 JUN 2010
  3. Manuscript Revised: 11 JUN 2010
  4. Manuscript Received: 6 OCT 2009

Funded by

  • Singapore-MIT Alliance and the National University of Singapore. Grant Number: R-279-000-249-646

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (776K)

Keywords:

  • chiral separation;
  • membrane separation;
  • human serum albumin;
  • selective permeation enhancement;
  • enantiomers

Abstract

Novel membrane processes for the effective enantioresolution of racemic mixtures have been evaluated. The incorporation of human serum albumin (HSA) in the strip solution of a permeation cell resulted in the partial optical resolution of a racemic tryptophan mixture, as the permeation of L-tryptophan, which binds to HSA more strongly, was enhanced selectively over that of D-tryptophan. A second approach in which a racemic mixture was introduced to the strip solution prior to the experiments showed better performance by selectively decreasing the flux of the more weakly bound D-tryptophan. The highest enantioselectivity of ∼9.76 was achieved with a third, novel design consisting of two permeation cells in series, which can encompass the advantages of affinity dialysis. An industrial scale unit is proposed based on this concept and a suitably validated mathematical model. © 2010 American Institute of Chemical Engineers AIChE J, 2011

View Full Article (HTML) Get PDF (776K)