Separations: Materials, Devices, and Processes

Modeling multicomponent adsorption of monoclonal antibody charge variants in cation exchange columns

  1. Yinying Tao1,†,
  2. Nianyun Chen1,‡,
  3. Giorgio Carta1,*,
  4. Gisela Ferreira2,
  5. David Robbins2

Article first published online: 29 DEC 2011

DOI: 10.1002/aic.13718

Issue

AIChE Journal

AIChE Journal

Volume 58, Issue 8, pages 2503–2511, August 2012

Additional Information

How to Cite

Tao, Y., Chen, N., Carta, G., Ferreira, G. and Robbins, D. (2012), Modeling multicomponent adsorption of monoclonal antibody charge variants in cation exchange columns. AIChE J., 58: 2503–2511. doi: 10.1002/aic.13718

Author Information

  1. 1

    Dept. of Chemical Engineering, University of Virginia, Charlottesville, VA 22904

  2. 2

    MedImmune, One MedImmune Way, Gaithersburg, MD 20878

  1. †

    Biogen Idec, Inc., 5000 Davis Drive, Research Triangle Park, NC 27709

  2. ‡

    Shire, 700 Main St. Cambridge, MA 02139

*Dept. of Chemical Engineering, University of Virginia, Charlottesville, VA 22904

Publication History

  1. Issue published online: 5 JUL 2012
  2. Article first published online: 29 DEC 2011
  3. Accepted manuscript online: 13 DEC 2011 09:51AM EST
  4. Manuscript Revised: 30 NOV 2011
  5. Manuscript Received: 24 AUG 2011

Funded by

  • MedImmune and NSF. Grant Number: CBET-1032727

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (216K)

Keywords:

  • monoclonal antibody charge variants;
  • ion exchange;
  • multicomponent adsorption kinetics;
  • mass transfer modeling;
  • diffusion

Abstract

Transport models are developed for the adsorption of monoclonal antibody charge variants in cation exchange columns for two different matrices: UNOsphere S, which has a macroporous architecture, and Capto S, which contains charged dextran polymers grafted to an agarose matrix. The UNOsphere S protein adsorption kinetics is described by a macropore diffusion model with effective pore diffusivity De = 8.3 × 10−8 cm2/s, which successfully predicts resolution of charge variants by frontal analysis at residence times, L/u, between 0.9 and 5.4 min. The Capto S adsorption kinetics is very fast for individual charge variants and for coadsorption of their mixtures, but is dramatically slower for sequential adsorption, when a more strongly bound variant displaces a more weakly bound one. A Maxwell-Stefan model assuming single-file diffusion predicts, in agreement with experiments, poor resolution of charge variants by frontal analysis even at long residence times as a result of highly hindered counterdiffusion. © 2011 American Institute of Chemical Engineers AIChE J, 58: 2503–2511, 2012

View Full Article (HTML) Get PDF (216K)