Isotherm selection for band profile simulations in preparative chromatography

Authors

  • Stephen Jacobson,

    1. Dept. of Chemistry, University of Tennessee, Knoxville, TN 37996
    2. Div. of Analytical Chemistry, Oak Ridge National Laboratory, Oak Ridge, TN 37831
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  • Sadroddin Golshan-Shirazi,

    1. Dept. of Chemistry, University of Tennessee, Knoxville, TN 37996
    2. Div. of Analytical Chemistry, Oak Ridge National Laboratory, Oak Ridge, TN 37831
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  • Georges Guiochon

    Corresponding author
    1. Dept. of Chemistry, University of Tennessee, Knoxville, TN 37996
    2. Div. of Analytical Chemistry, Oak Ridge National Laboratory, Oak Ridge, TN 37831
    • Dept. of Chemistry, University of Tennessee, Knoxville, TN 37996
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Abstract

Equilibrium adsorption data for the N-benzoyl derivatives of D- and L-phenyl alanine in water/propanol solutions have been determined on a chiral-selective stationary phase, bovine serum albumin immobilized on silica. These data are well accounted for by an isotherm equation resulting from the sum of two Langmuir terms. The first term corresponds to the chiral-selective interactions, and the second to the nonselective molecular interactions between the enantiomers and the stationary phase. The individual band profiles of the two enantiomeric amino acids, either pure or in binary mixtures of various compositions, are predicted exactly by an equilibrium dispersive model using this bi-Langmuir isotherm. A classical Langmuir isotherm fails to predict these band profiles, illustrating the importance to collect accurate adsorption data and to use a correct isotherm model when calculating chromatographic band profiles.

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