This study was supported in part by U.S.P.H.S. grants AM-11854 and 5T 01 GM-01548.
Effects of halothane on the ultrastructure of rat liver cells†
Version of Record online: 3 FEB 2005
Copyright © 1972 Wiley-Liss, Inc.
American Journal of Anatomy
Volume 135, Issue 1, pages 5–21, September 1972
How to Cite
Ross, W. T. and Cardell, R. R. (1972), Effects of halothane on the ultrastructure of rat liver cells. Am. J. Anat., 135: 5–21. doi: 10.1002/aja.1001350103
- Issue online: 3 FEB 2005
- Version of Record online: 3 FEB 2005
The objective was to study the effects of halothane, a volatile anesthetic, on hepatic metabolism of a second volatile anesthetic, methoxyflurane, and to correlate these biochemical findings with hepatic morphological changes. Microsomal fractions isolated from rats treated with halothane and from control animals were assayed for their capacity to dechlorinate methoxyflurane. Microsomes from halothane-treated rats demonstrated about 2.6 times the capacity to dechlorinate methoxyflurane as microsomes from control animals. Electron microscopy showed that liver cells from halothane-treated animals, when compared with hepatocytes from control rats, had increased amounts of smooth endoplasmic reticulum, an increased number of lipid droplets, and more microbodies per cell. Rough endoplasmic reticulum and glycogen were decreased by halothane treatment. We interpret these results to mean that halothane induces the rough endoplasmic reticulum to synthesize enzymes required for the biotransformation of methoxyflurane. It is suggested that these enzymes are placed in membranes of the rough endoplasmic reticulum. This organelle is converted to smooth endoplasmic reticulum and here the biotransforming enzymes function to dechlorinate methoxyflurane.