Cell surface of mouse blastocysts at the trophectoderm-uterine interface during the adhesive stage of implantation
Article first published online: 3 FEB 2005
Copyright © 1986 Wiley-Liss, Inc.
American Journal of Anatomy
Volume 176, Issue 2, pages 153–158, June 1986
How to Cite
Chávez, D. J. (1986), Cell surface of mouse blastocysts at the trophectoderm-uterine interface during the adhesive stage of implantation. Am. J. Anat., 176: 153–158. doi: 10.1002/aja.1001760205
- Issue published online: 3 FEB 2005
- Article first published online: 3 FEB 2005
- Manuscript Accepted: 14 FEB 1986
- Manuscript Received: 1 OCT 1985
The molecular basis for the acquisition of adhesiveness between blastocysts and uterine luminal epithelium is an interesting problem in reproductive biology. It is rather difficult to study implantation-stage blastocysts of mice because during the implantation period each blastocyst becomes lodged within a crypt formed by decidualizing stroma. After trophectoderm adheres to uterine luminal epithelium, it is not possible to flush intact blastocysts from the uterus by standard recovery procedures. By identifying implantation sites with the Evans blue technique and splitting or gently separating the apposed epithelium of finely trimmed sites, it was possible to expose nonadhesive and adhesive trophectoderm to polycationized ferritin (PCF) and a series of ferritin-conjugated lectins. Examination by transmission electron microscopy revealed that both adhesive and nonadhesive trophectoderm bound PCF, concanavalin A, wheat-germ agglutinin, Ricinus communis agglutinin I, and Limulus polyhemus agglutinin, but not Dolicos biflorus agglutinin or peanut agglutinin. Nonadhesive trophectoderm bound succinylated wheat germ agglutinin but adhesive trophectoderm did not. There was no apparent difference in the relative amounts of each lectin bound to adhering and nonadhering cells.