Decidual cells in the human ovary at term: II. Morphometric analysis of cytoplasmic processes and organelles
Article first published online: 3 FEB 2005
Copyright © 1987 Wiley-Liss, Inc.
American Journal of Anatomy
Volume 179, Issue 3, pages 277–284, July 1987
How to Cite
Herr, J. C., Fares, N. H. and Banhawy, M. A. (1987), Decidual cells in the human ovary at term: II. Morphometric analysis of cytoplasmic processes and organelles. Am. J. Anat., 179: 277–284. doi: 10.1002/aja.1001790309
- Issue published online: 3 FEB 2005
- Article first published online: 3 FEB 2005
- Manuscript Accepted: 11 OCT 1986
- Manuscript Received: 9 JUN 1986
Morphometric analysis of human ovarian decidual cells was performed with a Videoplan computer, and mean values were established for the area and perimeter of cellular processes and organelles. Two-hundred forty electron micrographs representing 160 cells were analyzed. The mean decidual cell area was 218.7 μm2 of which 34.5 μm2 was occupied by the nucleus (15.8% of the cytoplasmic area); the nucleus contained 1.74 μm2 of nucleolar material (0.8%). The endoplasmic reticulum occupied 13.63 μm2 (6.2%). Mitochondria occupied 7.3 μm2 (3.3%) and the Golgi network 5.49 μm2 (2.5%). Decidual secretory bodies occupied 0.91 μm2 (0.42%) and cytoplasmic processes 1.89 μm2 (0.94%). The remainder of the cytoplasm, containing inclusions and cytoskeleton, represented 71% of the cell area. Perimeter measurements indicated an average decidual cell was surrounded by 87.8 μm of plasma membrane. The mean nuclear membrane measured 28.3 μm (representing 32.3% of the plasma membrane, pm, or 4.1% of total cellular membranes, cm). Outer mitochondrial membranes measured 156.6 μm (178% pm, 23.5% cm); endoplasmic reticulum membranes measured 350.3 μm (400% pm, 52.6% cm); Golgi membrane measured 30.77 μm (35% pm; 4.5% cm) and membrane surrounding secretory bodies measured 9.8 μm (11.2% pm; 1.4% cm). A mean of 280 secretory bodies per ovarian decidual cell was calculated. The plasma membranes of evaginated cytoplasmic processes represented 22.3% of the total pm (19.6 μm or 2.9% cm). A mean of seven such processes was observed per 87.8 μm of plasma membrane (160/cell). These morphometric data provide a baseline for comparisons of human ovarian decidual cells with uterine decidua, in vivo and in vitro, as well as with decidual cells of other species.