Changes in the expression of fibroblast growth factor receptors mark distinct stages of chondrogenesis in vitro and during chick limb skeletal patterning
Article first published online: 3 FEB 2005
Copyright © 1995 Wiley-Liss, Inc.
Volume 204, Issue 4, pages 446–456, December 1995
How to Cite
Szebenyi, G., Savage, M. P., Olwin, B. B. and Fallon, J. F. (1995), Changes in the expression of fibroblast growth factor receptors mark distinct stages of chondrogenesis in vitro and during chick limb skeletal patterning. Dev. Dyn., 204: 446–456. doi: 10.1002/aja.1002040410
- Issue published online: 3 FEB 2005
- Article first published online: 3 FEB 2005
- Manuscript Accepted: 10 AUG 1995
- Manuscript Received: 20 JUN 1995
- FGF receptors;
- Limb development;
- Micromass cultures
Members of the fibroblast growth factor (FGF) family of growth factors are key regulators of limb skeletal patterning and growth. Abnormal expression of FGFs or mutations in their receptors (fgfrs) result in skeletal disorders. Here we show that changes in the expression of fgfrs are intrinsic properties of differentiating cartilage. In mesenchymal micromass cultures differentiating into cartilage, as in ovo, fgfr 1 mRNA was found predominantly in undifferentiated, proliferating mesenchyme, fgfr 2 in precartilage cell aggregates, and fgfr 3 in differentiating cartilage nodules. Thus, our data suggest that switches in the expression of fgfr 1, 2, and 3 mRNAs are associated with phases of cartilage patterning both in vitro and in ovo, and mark distinct stages in the development of the limb skeleton. © 1995 wiley-Liss, Inc.