Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow-based P-selectin-coated microtube

Authors


  • Conflict of Interest: MRK serves on the scientific advisory board of Cell-Traffix Inc., a company in which he holds financial interest.

Abstract

Recently, a flow-based selectin-dependent method for capture and enrichment of specific type blood cells (CD34+ hematopoietic stem and progenitor cells) from bone marrow and peripheral blood has been described. Using a similar approach, here we show isolation of CD45+ blood cells directly from human bone marrow to a high purity (90%–97%). The process mimics a ubiquitous mechanism of cell trafficking in the body for the recruitment of neutrophils during inflammation. The method is straightforward, rapid, and may represent a practical alternative to CD45+ cell enrichment procedures required for chimerism analysis following allogenic stem-cell transplantation. Am. J. Hematol., 2008. © 2008 Wiley-Liss, Inc.

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