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Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow-based P-selectin-coated microtube†

  1. Srinivas D. Narasipura1,
  2. Joel C. Wojciechowski1,2,
  3. Brian M. Duffy1,
  4. Jane L. Liesveld3,
  5. Michael R. King1,*

Article first published online: 10 APR 2008

DOI: 10.1002/ajh.21204

Issue

American Journal of Hematology

American Journal of Hematology

Volume 83, Issue 8, pages 627–629, August 2008

Additional Information

How to Cite

Narasipura, S. D., Wojciechowski, J. C., Duffy, B. M., L. Liesveld, J. and King, M. R. (2008), Purification of CD45+ hematopoietic cells directly from human bone marrow using a flow-based P-selectin-coated microtube. American Journal of Hematology, 83: 627–629. doi: 10.1002/ajh.21204

Author Information

  1. 1

    Department of Biomedical Engineering, University of Rochester, Rochester, New York

  2. 2

    CellTraffix Inc, Rochester, New York

  3. 3

    Department of Medicine, Wilmot Cancer Center, University of Rochester Medical Center, University of Rochester, Rochester, New York

*RC Box 270168, Department of Biomedical Engineering, University of Rochester, Rochester, NY, USA

  1. †

    Conflict of Interest: MRK serves on the scientific advisory board of Cell-Traffix Inc., a company in which he holds financial interest.

Publication History

  1. Issue published online: 14 JUL 2008
  2. Article first published online: 10 APR 2008
  3. Accepted manuscript online: 10 APR 2008 12:00AM EST
  4. Manuscript Accepted: 1 APR 2008
  5. Manuscript Revised: 25 MAR 2008
  6. Manuscript Received: 20 FEB 2008

Funded by

  • New York State Office of Science, Technology, Academic Research (NYSTAR)

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Abstract

Recently, a flow-based selectin-dependent method for capture and enrichment of specific type blood cells (CD34+ hematopoietic stem and progenitor cells) from bone marrow and peripheral blood has been described. Using a similar approach, here we show isolation of CD45+ blood cells directly from human bone marrow to a high purity (90%–97%). The process mimics a ubiquitous mechanism of cell trafficking in the body for the recruitment of neutrophils during inflammation. The method is straightforward, rapid, and may represent a practical alternative to CD45+ cell enrichment procedures required for chimerism analysis following allogenic stem-cell transplantation. Am. J. Hematol., 2008. © 2008 Wiley-Liss, Inc.

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