A flow cytometry-based assay for measuring invasion of red blood cells by Plasmodium falciparum

Authors

  • Amy K. Bei,

    1. Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts
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    • Amy K. Bei and Tiffany M. Desimone contributed equally to this work.

  • Tiffany M. DeSimone,

    1. Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts
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    • Amy K. Bei and Tiffany M. Desimone contributed equally to this work.

  • Aida S. Badiane,

    1. Laboratory of Bacteriology and Virology, Le Dantec Hospital, and Laboratory of Parasitology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Dakar BP 7325, Senegal
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  • Ambroise D. Ahouidi,

    1. Laboratory of Bacteriology and Virology, Le Dantec Hospital, and Laboratory of Parasitology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Dakar BP 7325, Senegal
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  • Tandakha Dieye,

    1. Laboratory of Bacteriology and Virology, Le Dantec Hospital, and Laboratory of Parasitology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Dakar BP 7325, Senegal
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  • Daouda Ndiaye,

    1. Laboratory of Bacteriology and Virology, Le Dantec Hospital, and Laboratory of Parasitology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Dakar BP 7325, Senegal
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  • Ousmane Sarr,

    1. Laboratory of Bacteriology and Virology, Le Dantec Hospital, and Laboratory of Parasitology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Dakar BP 7325, Senegal
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  • Omar Ndir,

    1. Laboratory of Bacteriology and Virology, Le Dantec Hospital, and Laboratory of Parasitology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Dakar BP 7325, Senegal
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  • Souleymane Mboup,

    1. Laboratory of Bacteriology and Virology, Le Dantec Hospital, and Laboratory of Parasitology, Faculty of Medicine and Pharmacy, Cheikh Anta Diop University, Dakar BP 7325, Senegal
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  • Manoj T. Duraisingh

    Corresponding author
    1. Harvard School of Public Health, 665 Huntington Avenue, Boston, Massachusetts
    • Harvard School of Public Health, 665, Huntington Avenue, Building 1, Room 715, Boston 02115, MA
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  • Conflict of interest: Nothing to report.

Abstract

Variability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry-based method for simultaneously calculating both the parasitemia and the number of multiply-infected erythrocytes. Staining with the DNA-specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion. Am. J. Hematol., 2010. © 2010 Wiley-Liss, Inc.

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