Erythropoietic precursors in mice with phenylhydrazine-induced anemia

Authors

  • Hiroshi Hara M.D.,

    1. Division of Hematology, Department of Medicine, School of Medicine of the University of South Carolina, and Veterans Administration Hospital, Charleston, South Carolina
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  • Makio Ogawa M.D., Ph.D.

    Corresponding author
    1. Division of Hematology, Department of Medicine, School of Medicine of the University of South Carolina, and Veterans Administration Hospital, Charleston, South Carolina
    • Veterans Administration Hospital, 109 Bee Street, Charleston, South Carolina 29403
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Abstract

Using a methylcellulose cell culture technique, we studied the serial changes in erythropoietic precursors in the femur, spleen, and blood of mice under erythropoietic stimuli. Phenylhydrazine hydrochloride, in the dosage of 60 mg/kg, was injected into mice subcutaneously on days 0, 1, and 3, and mice were sacrificed on days 0, 2, 4, 7, and 10 for assessment of erythropoietic precursors. Significant changes were observed for all hemopoietic organs in the number of erythrocytic burst-forming units (BFU-E) and erythrocytic colony-forming units (CFU-E). Only BFU-E were present in blood, and their maximal increase was noted on day 2. While marrow BFU-E continuously decreased, maximal increase of CFU-E noted on day 4. Splenic BFU-E and CFU-E increased until day 4 and declined subsequently. These observations suggest the presence of significant migration of BFU-E in mice under erythropoietic stimuli and stress the importance of studies on all hemopoietic organs in the assessment of murine hemopoiesis.

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