Multimeric pattern of plasma and platelet von willebrand factor is normal in uremic patients

Authors

  • Giancarlo Castaman,

    1. Department of Hematology and Hemophilia and Thrombosis Center and Department of Nephrology, San Bortolo Hospital, Vicenza and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, IRCCS Maggiore Hospital and University of Milano, Italy
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  • Dr. Francesco Rodeghiero,

    Corresponding author
    1. Department of Hematology and Hemophilia and Thrombosis Center and Department of Nephrology, San Bortolo Hospital, Vicenza and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, IRCCS Maggiore Hospital and University of Milano, Italy
    • Department of Hematology and Hemophilia and Thrombosis Center, San Bortolo Hospital, 36100 Vicenza, Italy
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  • Antonella Lattuada,

    1. Department of Hematology and Hemophilia and Thrombosis Center and Department of Nephrology, San Bortolo Hospital, Vicenza and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, IRCCS Maggiore Hospital and University of Milano, Italy
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  • Giuseppe La Greca,

    1. Department of Hematology and Hemophilia and Thrombosis Center and Department of Nephrology, San Bortolo Hospital, Vicenza and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, IRCCS Maggiore Hospital and University of Milano, Italy
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  • Pier Mannuccio Mannucci

    1. Department of Hematology and Hemophilia and Thrombosis Center and Department of Nephrology, San Bortolo Hospital, Vicenza and Angelo Bianchi Bonomi Hemophilia and Thrombosis Center, IRCCS Maggiore Hospital and University of Milano, Italy
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Abstract

Several studies have reported increased von Willebrand factor (vWF) levels in plasma of uremic patients, with a normal multimeric pattern. Two recent studies, however, have shown a reduction of the larger multimers of plasma vWF and one has also found low levels of platelet vWF in uremic patients with prolonged bleeding time (BT). We have measured plasma and platelet vWF and analyzed its multimeric pattern in 20 uremic patients, 11 with a prolonged BT and 9 with a normal BT. For Plasma vWF, no difference for vWF:Ag, RiCof, and ristocetin-induced platelet agglutination was found between the two groups with normal and prolonged BT. The multimeric pattern of plasma vWF, as evaluated by densitometric scanning of the electrophoretic gels, was normal in both groups. For platelet vWF, vWF:Ag and RiCof content was similar in the two groups. The multimeric pattern was indistinguishable from that of normal platelets. In conclusion, our study does not confirm the presence of a structural defect of plasma vWF and the reduction of platelet vWF content in uremia. © 1993 Wiley-Liss, Inc.

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