Assessing interethnic admixture using an X-linked insertion-deletion multiplex

Authors

  • Elzemar Martins Ribeiro-Rodrigues,

    1. Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brazil
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  • Ney Pereira Carneiro dos Santos,

    1. Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brazil
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  • Ândrea Kely Campos Ribeiro dos Santos,

    1. Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brazil
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  • Rui Pereira,

    1. IPATIMUP, Instituto de Patologia e Imunologia Molecular da Universidade do Porto, 4200-465 Porto, Portugal
    2. Institute of Legal Medicine, University of Santiago de Compostela E-15782, Santiago de Compostela, Spain
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  • António Amorim,

    1. IPATIMUP, Instituto de Patologia e Imunologia Molecular da Universidade do Porto, 4200-465 Porto, Portugal
    2. Faculdade de Ciências da Universidade do Porto, 4050 Porto, Portugal
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  • Leonor Gusmão,

    1. IPATIMUP, Instituto de Patologia e Imunologia Molecular da Universidade do Porto, 4200-465 Porto, Portugal
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  • Marco Antonio Zago,

    1. Faculdade de Medicina de Ribeirão Preto, Departamento de Clínica Médica, Universidade de São Paulo, São Paulo, Brazil
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  • Sidney Emanuel Batista dos Santos

    Corresponding author
    1. Laboratório de Genética Humana e Médica, Departamento de Patologia, Universidade Federal do Pará, Belém, Pará, Brazil
    • Universidade Federal do Pará, Centro de Ciências Biológicas, Laboratório de Genética Humana e Médica, Rua Augusto Corrêa 01, Guamá, Caixa Postal 8615, CEP: 66075-970 Belém, Pará, Brasil
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Abstract

In this study, a PCR multiplex was optimized, allowing the simultaneous analysis of 13 X-chromosome Insertion/deletion polymorphisms (INDELs). Genetic variation observed in Africans, Europeans, and Native Americans reveals high inter-population variability. The estimated proportions of X-chromosomes in an admixed population from the Brazilian Amazon region show a predominant Amerindian contribution (≅41%), followed by European (≅32%) and African (≅27%) contributions. The proportion of Amerindian contribution based on X-linked data is similar to the expected value based on mtDNA and Y-chromosome information. The accuracy for assessing interethnic admixture, and the high differentiation between African, European, and Native American populations, demonstrates the suitability of this INDEL set to measure ancestry proportions in three-hybrid populations, as it is the case of Latin American populations. Am. J. Hum. Biol. 2009. © 2009 Wiley-Liss, Inc.

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