Measurement of testosterone in infant fecal samples
Article first published online: 19 JUL 2011
Copyright © 2011 Wiley-Liss, Inc.
American Journal of Human Biology
Volume 23, Issue 6, pages 820–822, November/December 2011
How to Cite
Thompson, A. L., Whitten, P. L. and Lampl, M. (2011), Measurement of testosterone in infant fecal samples. Am. J. Hum. Biol., 23: 820–822. doi: 10.1002/ajhb.21205
- Issue published online: 10 OCT 2011
- Article first published online: 19 JUL 2011
- Manuscript Accepted: 13 JUN 2011
- Manuscript Revised: 19 MAY 2011
- Manuscript Received: 14 JAN 2011
- National Science Foundation (Dissertation Improvement Grant). Grant Number: NSF BCS# 0424307
- Robert H. Woodruff Health Sciences Center, Emory University
This study reports the validation of a noninvasive method for repeated assessment of testosterone from infant fecal samples.
Fecal samples were collected from cotton diaper liners, subjected to methanol extraction, and assayed using a modified commercial testosterone RIA kit.
Method validity was supported by a recovery near 100%, a sensitivity of 1.23 pg/ml, and inter- and intra-assay coefficients of variations less than 10 and 15%, respectively. Testosterone was detected in all samples from male and female infants aged 2 weeks to 15 months.
Fecal assessment is supported as a novel, non-invasive tool for studying testosterone during early human development. Am. J. Hum. Biol., 2011. © 2011 Wiley-Liss, Inc.