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Abstract

Objectives:

This study reports the validation of a noninvasive method for repeated assessment of testosterone from infant fecal samples.

Methods:

Fecal samples were collected from cotton diaper liners, subjected to methanol extraction, and assayed using a modified commercial testosterone RIA kit.

Results:

Method validity was supported by a recovery near 100%, a sensitivity of 1.23 pg/ml, and inter- and intra-assay coefficients of variations less than 10 and 15%, respectively. Testosterone was detected in all samples from male and female infants aged 2 weeks to 15 months.

Conclusions:

Fecal assessment is supported as a novel, non-invasive tool for studying testosterone during early human development. Am. J. Hum. Biol., 2011. © 2011 Wiley-Liss, Inc.