Sampling strategies in a linguistic isolate: Results from mtDNA analysis
Article first published online: 27 JAN 2012
Copyright © 2012 Wiley Periodicals, Inc.
American Journal of Human Biology
Volume 24, Issue 2, pages 192–194, March/April 2012
How to Cite
Calò, C. M., Corrias, L., Vona, G., Bachis, V. and Robledo, R. (2012), Sampling strategies in a linguistic isolate: Results from mtDNA analysis. Am. J. Hum. Biol., 24: 192–194. doi: 10.1002/ajhb.22215
- Issue published online: 8 FEB 2012
- Article first published online: 27 JAN 2012
- Manuscript Accepted: 22 NOV 2011
- Manuscript Revised: 3 OCT 2011
- Manuscript Received: 11 JUL 2011
- Italian Ministry of University PRIN 2007. Grant Number: TYXE3X
Sampling strategies are crucial issues in population genetics and anthropological studies. The sampling choice is related to the research question and the type of markers used. In this research, we compared two different sampling strategies in the Sardinian linguistic isolate of Carloforte (Italy).
A first sampling (N = 49) was carried out through grandparents criterion: individuals selected for the study were born and resident in Carloforte, and unrelated for at least three generations. A second sampling (N = 50) was based on founders surnames (FS): selected participants were proved to be descendants of the village founders, and to have no ancestors in common, at least up to the grandparental generation.
The group selected through FS showed a greater gene diversity, which was confirmed by both network and haplogroup analysis. Among the shared haplogroups, we find clear differences in their frequencies. Sampling through grandparents criterion showed essentially the same haplogroups found in Sardinia, and with similar frequencies. Interesting results came from genetic tree. The FS sampling clustered with Northern African populations and it is located very far from Italian and Sardinian populations, whereas the grandparents criterion sampling clustered with Italian populations and it is located close to the other Sardinian populations.
Results showed that different sampling strategies can lead to contrasting results. As sampling through grandparents criterion is influenced by recent gene flow, we hypothesize that the difference observed with the two sampling strategies is due to the merging of Carloforte with Sardinian populations. Am. J. Hum. Biol., 2012. © 2012 Wiley Periodicals, Inc.