C.E.V.W., M.R.L., and S.M.B.S. contributed equally to this work.
Y-Linked microsatellites in Amazonian Amerindians applied to ancestry estimates in Brazilian Afro-derived populations
Article first published online: 24 JAN 2013
Copyright © 2013 Wiley Periodicals, Inc.
American Journal of Human Biology
Volume 25, Issue 3, pages 313–317, May/June 2013
How to Cite
Wiezel, C. E.V., Luizon, M. R., Sousa, S. M.B., Santos, L. M.W., Muniz, Y. C.N., Mendes-Junior, C. T. and Simões, A. L. (2013), Y-Linked microsatellites in Amazonian Amerindians applied to ancestry estimates in Brazilian Afro-derived populations. Am. J. Hum. Biol., 25: 313–317. doi: 10.1002/ajhb.22361
- Issue published online: 20 APR 2013
- Article first published online: 24 JAN 2013
- Manuscript Accepted: 19 NOV 2012
- Manuscript Revised: 2 OCT 2012
- Manuscript Received: 9 MAY 2012
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brazil); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brazil)
Proper ancestral populations are required to determine accurate ancestry estimates for Afro-derived Brazilian populations. Herein, we have genotyped Y-STRs in Amazonian Amerindians to determine the ancestral contribution in quilombo remnant communities.
The frequencies for five Y-chromosome linked microsatellites (DYS19, DYS390, DYS391, DYS392, and DYS393) were characterized in four Amerindian tribes from Brazilian Amazon (Tikúna, Baníwa, Kashinawa, and Kanamarí), and in four quilombo remnants (Mimbó, Sítio Velho, Gaucinha, and São Gonçalo) and two urban populations (Teresina and Jequié) from Northeastern Brazil. We then estimated the male genetic ancestry in each admixed population. Moreover, we performed analysis of molecular variance (AMOVA), FST, haplotype diversity, and principal component analysis.
Lower haplotype diversity (h) values were observed for Tikúna compared with other tribes. Quilombo remnants exhibited higher h levels ranging from 0.893 ± 0.027 in Sítio Velho to 0.963 ± 0.033 in São Gonçalo. African ancestry estimates ranged from 0.529 ± 0.027 in Mimbó to 0.602 ± 0.086 in Sítio Velho. Conversely, European contribution was 0.795 ± 0.045 in Teresina and 0.826 ± 0.040 in Jequié.
FST and principal component analysis indicate homogeneity in the male genetic constitution among the quilombo remnants analyzed. Data on Amerindians allowed accurate ancestry estimates, which indicated a higher African contribution, followed by a considerable European contribution for these quilombo remnants. Am. J. Hum. Biol. 25:313–317, 2013. © 2013 Wiley Periodicals, Inc.