B. Laffon and J.P. Teixeira contributed equally to this work.
Genotoxic effects in a population of nurses handling antineoplastic drugs, and relationship with genetic polymorphisms in DNA repair enzymes†
Article first published online: 19 JUL 2005
Copyright © 2005 Wiley-Liss, Inc.
American Journal of Industrial Medicine
Volume 48, Issue 2, pages 128–136, August 2005
How to Cite
Laffon, B., Teixeira, J.P., Silva, S., Loureiro, J., Torres, J., Pásaro, E., Méndez, J. and Mayan, O. (2005), Genotoxic effects in a population of nurses handling antineoplastic drugs, and relationship with genetic polymorphisms in DNA repair enzymes. Am. J. Ind. Med., 48: 128–136. doi: 10.1002/ajim.20189
- Issue published online: 19 JUL 2005
- Article first published online: 19 JUL 2005
- Manuscript Accepted: 2 MAY 2005
- The Xunta de Galicia (postdoctoral fellowship to B. Laffon). Grant Numbers: PGIDT01PXI10602PR, PR409A2002/169-0
- antineoplastic drugs;
- genotoxic damage;
- comet assay;
- genetic polymorphisms;
- DNA repair;
Concern about the genotoxic risk associated with chronic handling of antineoplastic drugs has increased, and usual safety practices may not avoid exposure.
Comet assay and MN test were performed on 30 oncology nurses and 22 controls. Genetic polymorphisms of XRCC1, XRCC3, and APE1 genes were determined by PCR-RFLP.
Data obtained showed increased cytogenetic and DNA damage in the exposed group, although statistical significance was only reached in the comet assay. Significant differences in TL were observed for carriers of the variant alleles of every gene analyzed. However, no significant effect was detected in the MN test.
Evidence that the present handling practices of antineoplastic drugs in some Portuguese hospitals are not enough to prevent exposure are provided. Present data suggest that genetic polymorphisms in the studied DNA repair enzymes may influence the individual susceptibility to DNA damage related to chronic handling of antineoplastic drugs. Am. J. Ind. Med. 48:128–136, 2005. © 2005 Wiley-Liss, Inc.