External validation of recombinant antigens for serodiagnosis of machine operator's lung

Authors

  • Coralie Barrera MSc,

    1. UMR/CNRS 6249 Chrono-Environnement, University of Franche-Comté, Besançon, France
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  • Gabriel Reboux PhD,

    Corresponding author
    1. UMR/CNRS 6249 Chrono-Environnement, University of Franche-Comté, Besançon, France
    2. Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France
    • Correspondence to: Gabriel Reboux, PhD, Parasitology-Mycology Department, University Hospital of Besançon, Bd Fleming, 25 000 Besançon, France. E-mail: gabriel.reboux@univ-fcomte.fr

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  • Isabel Warfolomeow PhD,

    1. Occupational Health and Safety Department, Berufsgenossenschaft Holz und Metall (BGHM), Mainz, Germany
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  • Bénédicte Rognon PhD,

    1. UMR/CNRS 6249 Chrono-Environnement, University of Franche-Comté, Besançon, France
    2. Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France
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  • Laurence Millon PharmD, PhD,

    1. UMR/CNRS 6249 Chrono-Environnement, University of Franche-Comté, Besançon, France
    2. Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France
    3. Clinical Investigation Center (Inserm CIT 808), University Hospital of Besançon, Besançon, France
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  • Sandrine Roussel PhD

    1. UMR/CNRS 6249 Chrono-Environnement, University of Franche-Comté, Besançon, France
    2. Parasitology-Mycology Department, University Hospital of Besançon, Besançon, France
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  • Disclosure Statement: The authors report no conflicts of interests.

Abstract

Background

Machine operator's lung (MOL) is a hypersensitivity pneumonitis the diagnosis of which is difficult. Our laboratory previously developed an ELISA test using recombinant antigens from Mycobacterium immunogenum isolated in French plant. The objective was to validate the previous ELISA results with ten new suspected cases from Germany.

Methods

Two serological analyses were performed: ELISA with the six recombinant antigens, and electrosyneresis with crude antigens of M. immunogenum and three other main species isolated from contaminated metalworking fluids.

Results

The two recombinant antigens acyl-CoA dehydrogenase and dihydrolipoyl dehydrogenase, combined together, and electrosyneresis are useful in making the diagnosis regardless of the clinical and radiological data. Finally 9 out of the 10 suspected cases were declared as MOL.

Conclusions

Despite the geographical distance, the crude and recombinant antigens produced to investigate the clustered French cases also proved to be useful in diagnosing the suspected cases in Germany. Am. J. Ind. Med. 57:195–201, 2014. © 2013 Wiley Periodicals, Inc.

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