Rapid Publication Article

Human cardiac myosin heavy chain gene mapped within chromosome region 14q11.2→q13

  1. Rumiko Matsuoka1,*,
  2. Michihiro C. Yoshida4,
  3. Naotoshi Kanda2,
  4. Misa Kimura1,
  5. Hisashi Ozasa3,
  6. Atsuyoshi Takao1,
  7. John M. Opitz Editor,
  8. James F. Reynolds Editor

Article first published online: 5 JUN 2005

DOI: 10.1002/ajmg.1320320234

Issue

American Journal of Medical Genetics

American Journal of Medical Genetics

Volume 32, Issue 2, pages 279–284, February 1989

Additional Information

How to Cite

Matsuoka, R., Yoshida, M. C., Kanda, N., Kimura, M., Ozasa, H., Takao, A., Opitz, J. M. and Reynolds, J. F. (1989), Human cardiac myosin heavy chain gene mapped within chromosome region 14q11.2→q13. Am. J. Med. Genet., 32: 279–284. doi: 10.1002/ajmg.1320320234

Author Information

  1. 1

    Department of Pediatric Cardiology, The Heart Institute of Japan

  2. 2

    Department of Anatomy Chromosome Research Unit, Hokkaido University, Japan

  3. 3

    Department of Kidney Center Chromosome Research Unit, Hokkaido University, Japan

  4. 4

    Tokyo Women's Medical College, and Chromosome Research Unit, Hokkaido University, Japan

*Department of Pediatric Cardiology, The Heart Institute of Japan, Tokyo Women's Medical College, 8-1 Kawada-cho Shinjuku-ku, Tokyo 162, Japan

Publication History

  1. Issue published online: 5 JUN 2005
  2. Article first published online: 5 JUN 2005

Funded by

  • Open Research
  • Japan Research Promotion Society for Cardiovascular Diseases, a Research Grant for Cardiovascular Diseases. Grant Number: 62-3
  • Ministry of Education and Science of Japan. Grant Number: GI570473

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Keywords:

  • regional mapping;
  • in situ hybridization;
  • contractile protein genes;
  • muscle;
  • myosin heavy-chain gene

Abstract

Our previous report demonstrated that two human cardiac α- and β-myosin heavy-chains (MHCs) which correspond to MYH6 and MYH7 respectively, according to Human Gene Mapping nomenclature, were mapped to human chromosome 14 and that human cardiac and skeletal MHC genes do not cosegregate. For further analysis, the regional mapping method was used. DNA from 4 human deletion and 3 human duplication cell lines were prepared for southern blotting, hybridized with human cardiac α- and β-MHC DNA probes, and the hybridization intensity relative to 46,XX or 46,XY DNA was estimated. The results showed that two human cardiac MHC genes segregated with the 14cen→q13 region of the long arm of human chromosome 14. In situ hybridization of 3H-labeled human cardiac α-MHC probe to normal human metaphase chromosome independently confirmed this result.

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