PCR detection of distal Yp sequences in an XX true hermaphrodite

Authors

  • Dr. Yosuo Nakagome,

    Corresponding author
    1. Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo
    • National Children's Meical Research Center, 3-35-31 Taishido, Setagaya-ku, Tokyo 154, Japan
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  • Satoko Seki,

    1. Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo
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  • Dr. Keiko Fukutani,

    Corresponding author
    1. Department of Urology, University of Tokyo Branch Hospital, Tokyo
    • Department of Urology, Asoka Hospital, Tokyo
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  • Shigeo Nagafuchi,

    1. Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo
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  • Yutaka Nakahori,

    1. Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo
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  • Takashi Tamura

    1. Department of Congenital Abnormalities Research, National Children's Medical Research Center, Tokyo
    2. Department of Clinical Genetics, School of Health Sciences, Kyorin University, Tokyo
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Abstract

An XX true hermaphrodite was examined for the presence of Y-specific sequences using Southern-blotting and polymerase chain reaction (PCR) techniques. of 25 loci examined, only two, the proximal border of the pseudoautosomal region (PABY) and the sex determining region of the Y chromosome (SRY), were detected. A crossing over event in paternal meiosis, proximal to the SRY locus but distal to the zinc finger protein (ZFY) locus, presumably transferred to two loci to the chromosome.

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