Polymorphisms in the CBS gene and homocysteine, folate and vitamin B12 levels: Association with polymorphisms in the MTHFR and MTRR genes in Brazilian children

Authors

  • Ana C.M. Aléssio,

    1. Faculty of Medical Sciences, Hematology-Hemotherapy Center, State University of Campinas (UNICAMP), Campinas, SP, Brazil
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  • Lúcia H. Siqueira,

    1. Faculty of Medical Sciences, Hematology-Hemotherapy Center, State University of Campinas (UNICAMP), Campinas, SP, Brazil
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  • Sérgio P. Bydlowski,

    1. Hematology-Hemotherapy Center, University of São Paulo, São Paulo, SP, Brazil
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  • Nelci F. Höehr,

    1. Faculty of Medical Sciences, Department of Clinical Pathology, State University of Campinas (UNICAMP), Campinas, SP, Brazil
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  • Joyce M. Annichino-Bizzacchi

    Corresponding author
    1. Faculty of Medical Sciences, Hematology-Hemotherapy Center, State University of Campinas (UNICAMP), Campinas, SP, Brazil
    • Hematology-Hemotherapy Center/State University of Campinas (UNICAMP), P.O. Box 6198, 13083-970 Campinas, SP, Brazil.
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  • How to cite this article: Aléssio ACM, Siqueira LH, Bydlowski SP, Höehr NF, Annichino-Bizzacchi JM. 2008. Polymorphisms in the CBS gene and homocysteine, folate and vitamin B12 levels: Association with polymorphisms in the MTHFR and MTRR genes in Brazilian children. Am J Med Genet Part A 146A:2598–2602.

Abstract

Polymorphisms in the methylenetetrahydrofolate reductase (MTHFR), methionine synthase reductase (MTRR) and cystathionine β-synthase (CBS) genes, involved in the intracellular metabolism of homocysteine (Hcy), can result in hyperhomocysteinemia. The objective of this study was to evaluate prevalence estimates of CBS T833C, G919A and the insertion of 68-bp (844ins68) polymorphisms and their correlation with Hcy, folate and B12 in 220 children previously genotyped for MTHFR C677T, A1298C, and MTRR A66G. The prevalence of heterozygote children for 844ins68 was 19.5%. The T833C CBS mutation was identified in association with 844ins68 in all the carriers of the insertion. Genotyping for CBS G919A mutation showed that all the children presented the GG genotype. Analysis of Hcy, B12 and folate, according to the combination of the different genotypes of the C677T and A1298C MTHFR, A66G MTRR, and 844ins68 CBS showed that the 677TT/1298AA/68WW genotype is associated with an increase in Hcy, when compared to the 677CC/1298AC/68WW (P = 0.033) and the 677CT/1298AA/68WW genotypes (P = 0.034). Since B12 and folate were not different between these groups, a genetic interaction between diverse polymorphisms probably influences Hcy. Our results emphasize the role of genetic interactions in Hcy levels. © 2008 Wiley-Liss, Inc.

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