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AJMA_33980_sm_Suppl-Fig-1.tif3454KFig. 1: RT-PCR expression of WDR65 and Wdr65. The RT-PCR of WDR65 (A) and Wdr65 (B, C, D) with primers specifically designed for the long (WDR65) and short (WDR65b) transcripts. In adult tissues, both human (A) and murine (B) long transcripts show the highest expression in testis, lung, brain, and skin. In murine embryos (C), the expression of Wdr65 parallels Irf6, but is delayed. Whereas Irf6 is expressed strongly in embryonic stem cells (Emb. SC), then disappears by 7 dpc and reappears at 11 dpc, Wdr65 is detectable in embryonic stem cells, then disappears by 7 and reappears at 15 dpc. (D) Expression in whole palates and medial edge epithelium (MEE) is shown for the Wdr65 transcripts and for Irf6. Again, expression of Wdr65 and Wdr65b transcripts parallel Irf6, but are delayed in peak expression. Also, Wdr65 expression is absent in Irf6-deficient embryos. RPL4 and Rpl4 are positive controls for human and mouse, respectively.
AJMA_33980_sm_Suppl-Fig-2.tif3642KFig. 2: Multiple Sequence Alignment of WDR65 and Wdr65. The amino acids of the long protein of WDR65 human and mouse aligned by T-coffee and formatted by Boxshade. Amino acid residues are identical (black), similar (gray), and divergent (white). The WD repeat domains are underlined in black.
AJMA_33980_sm_Suppl-Fig-3.tif1789KFig. 3: Multiple Sequence Alignment of WDR65b and Wdr65b. The amino acids of the short isoform of WDR65b human and mouse aligned by T-coffee and formatted by Boxshade. Amino acid residues are identical (black), similar (gray), and divergent (white). The WD repeat domains are underlined in black.
AJMA_33980_sm_Suppl-Table-I.doc107KTable I: Primers Used for Expression and Sequence Analyses
AJMA_33980_sm_Suppl-Table-II.doc62KTable II: Genetic Variants Identified in WDR65

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