How to Cite this Article: Li L, Zhu G-q, Meng T, Shi J-y, Wu J, Xu X, Shi B. 2011. Biological and epidemiological evidence of interaction of infant genotypes at Rs7205289 and maternal passive smoking in cleft palate. Am J Med Genet Part A 155: 2940–2948.
Biological and epidemiological evidence of interaction of infant genotypes at Rs7205289 and maternal passive smoking in cleft palate†
Article first published online: 19 OCT 2011
Copyright © 2011 Wiley Periodicals, Inc.
American Journal of Medical Genetics Part A
Volume 155, Issue 12, pages 2940–2948, December 2011
How to Cite
Li, L., Zhu, G.-q., Meng, T., Shi, J.-y., Wu, J., Xu, X. and Shi, B. (2011), Biological and epidemiological evidence of interaction of infant genotypes at Rs7205289 and maternal passive smoking in cleft palate. Am. J. Med. Genet., 155: 2940–2948. doi: 10.1002/ajmg.a.34254
- Issue published online: 21 NOV 2011
- Article first published online: 19 OCT 2011
- Manuscript Accepted: 17 JUL 2011
- Manuscript Received: 15 MAY 2011
- National Science Funds of China. Grant Number: 81070816
- State Key Laboratory of Oral Diseases. Grant Number: 2010.1-2011.12
Additional Supporting Information may be found in the online version of this article.
|ajmg_3454_sm_SupplFigS1.tif||121K||Fig. S1: Apparatus used for preparing cigarette smoke extract (CSE) solution. Mainstream smoke from commercial cigarettes was drawn through 35 ml PBS by using a 50 ml syringe. To generate CSE, stopcock (1) was opened and stopcock (2) was closed when the pump inspired; stopcock (1) was closed and stopcock (2) was opened when the pump expired.|
|ajmg_3454_sm_SupplFigS2.tif||4631K||Fig. S2: miR-140 expression in mouse palatal shelves. At E12 and E13, miR-140 was expressed in vertical palatal shelves (arrowheads in A, B); at E14, miR-140 was expressed in horizontal palatal shelves (arrowheads in C); at E15, and miR-140 was expressed in fused palatal shelves (arrowheads in D). No signal was detected using a scrambled miRNA (negative control, E). U6 RNA (positive control) was expressed ubiquitously in the tissue (F). br, brain; p, palatal shelf; t, tongue.|
|ajmg_3454_sm_SupplFigS3.tif||6314K||Fig. S3: Fluorescence in situ hybridization (FISH) of miR-140 in mouse palatal mesenchymal cells (MPMCs). The nuclei were stained by DAPI (blue fluorescent signal). miR-140 expression (green fluorescent signal) was detected from 6 to 48 hours of culture time and located in the cytoplasm (a-l). The probe for U6 RNA (positive control) hybridized most extensively to nuclear site with some cytoplasmic signal (m-o). No signal was detected when cells were hybridized with a scrambled probe (negative control, p-r).|
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